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KMID : 0364820140500010067
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Korean Journal of Microbiology
2014 Volume.50 No. 1 p.67 ~ p.72
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Isolation and Characterization of Nicotine-Degrading Bacterium Arthrobacter sp. NU11 and NU15
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Jeong Yeon-Ju
Oh Ji-Sung
Roh Dong-Hyun
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Abstract
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Minimal broth containing nicotine as a sole carbon source (MB/N) was used to isolate novel nicotine-degrading bacterial strains from tobacco plants and field soils. Comparative analysis of 16S rRNA gene sequence, phenotypic test and morphological tests showed that the position of these isolates were in the genus Arthrobacter of the family Micrococcaceae. The highest 16S rRNA gene sequence similarity of the isolate NU11 and NU15 to type strains in the genus Arthrobacter were Arthrobacter equi (98.2%) which was presumably a novel strain and Arthrobacter nicotinovorans (99.8%), respectively. Both strain NU11 and NU15 showed rod shaped, Gram-positive characteristics and catalase activity, but did not show oxidase activity. The novel strain NU11 was found to degrade efficiently nicotine in MB/N medium by the analysis of UV absorption spectra and could be used as an organism in bioremediation technique
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KEYWORD
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Arthrobacter sp. characterization, nicotine degrading bacteria
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